Fig 1: Expression of CFL1 in M. avium-infected macrophages. (A) Expression of CFL1 mRNA in macrophages infected with M. avium as determined via reverse transcription-quantitative PCR. (B) The concentration of CFL1 in the supernatant of macrophages infected with M. avium was measured by ELISA. (C and D) The expression levels of CFL1 protein in macrophages were detected via western blotting. *P<0.05, **P<0.005, ***P<0.001 vs. NC. M. avium, Mycobacterium avium. NC, negative control; CFL1, cofilin-1.
Fig 2: Receiver operating characteristic (ROC) curves show the diagnostic ability of CFL1 to distinguish patients with TB infection from healthy controls. (A) CFL1 mRNA expression in PBMCs to distinguish TB infection from healthy controls. (B) CFL1 protein expression in plasma to distinguish TB from healthy controls. AUC values between 90 and 100% are considered excellent, 80-90% good, 70-80% fair, 60-70% poor, 50-60% bad classifiers. Area under the ROC curve (AUC) <50% indicates random values that are not capable of distinguishing between two groups. TB, tuberculosis; PBMCs, peripheral blood mononuclear cells; CFL1, cofilin-1.
Fig 3: Expression of CFL1 in macrophages is influenced by M. avium infection (TB). (A) Expression of CFL1 mRNA in macrophages was regulated by CFL1 and M. avium (TB) as determined via reverse transcription-quantitative PCR. (B) CFL1 protein expression in the supernatant of M. avium-infected macrophages as measured by ELISA. (C and D) CFL1 protein expression levels in CFL1-siRNA transfected macrophages or M. avium-infected cells were detected via western blotting. Scrambled siRNA, cofilin-1 siRNA, scrambled siRNA + TB, cofilin-1 siRNA vs. NC. *P<0.05, **P<0.005, ***P<0.001 vs. NC. NC, normal control; M. avium, Mycobacterium avium; siRNA, small interfering RNA; CFL1, cofilin-1; TB, tuberculosis.
Fig 4: Expression levels of CFL1 in patients infected with M. avium infection (TB) were quantified. (A) CFL1 mRNA expression levels in the PBMCs of patients infected with TB were detected via reverse transcription-quantitative PCR. (B) The concentration of CFL1 protein in the plasma of patients infected with TB was measured by ELISA. (C) The relative expression of CFL1 protein in PBMCs of patients infected with TB was determined via western blotting. **P<0.005, ***P<0.001. vs. Normal cases. (D and E) Partial results of western blotting experiments of CFL1 protein in PBMCs of patients infected with TB were performed and the bands were statistically analyzed. TB cases (13#, 21#, 27#, and 31#) vs. normal cases (N, 1#, 16#, 29#). ***P<0.001 vs. Normal. TB, tuberculosis; PBMCs, peripheral blood mononuclear cells; CFL1, cofilin-1.
Supplier Page from CUSABIO Technology LLC for Human Cofilin-1(CFL1) ELISA Kit